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. 2021 Feb 12;11:547102. doi: 10.3389/fimmu.2020.547102

Table 3.

Media components and source.

Item Catalog Solvent Final Concentration
Advanced DMEM/F12 ThermoFisher Scientific 12634
GlutaMAX ThermoFisher Scientific 35050 1x
Hepes 7.3 ThermoFisher Scientific 15630 1x
Pen/Strep ThermoFisher Scientific 15140 1x
B-27 Supplement ThermoFisher Scientific 17504 1x
N-2 Supplement ThermoFisher Scientific 17502 1x
N-Acetylcysteine Sigma A9165 Distilled H20 10 uM
Human EGF ThermoFisher Scientific PMG8043 PBS/0.1% BSA 50 ng/ml
Mouse Noggin Peprotech 250-38 PBS/0.1% BSA 100 ng/ml
Human R-Spondin-1 R&D 4645-CF PBS/0.1% BSA 500 ng/ml
Mouse Wnt3a R&D 1324-CF PBS/0.1% BSA 100 ng/ml
ROCK Inhibitor (Y-27632) Sigma Y0503 PBS 10 uM
TGF-βR Inhibitor (SB-431542) Tocris 1614 DMSO 10 uM
FBS Sigma F2442
Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, *LDEV-Free, 10mL Corning 356231
Nicotinamide Sigma N0636 PBS 10 uM
Gastrin Sigma G9145 PBS 50 nM
P38 inhibitor (SB-202190) Tocris 1264 DMSO 10 uM