Fig. 4.

FGFRis decrease migration and invasion of BC cells. (a) MDA-MB-231(SA) and MFM223 cells were subjected to scratch wound migration assays in the presence of FGFRis at the indicated concentrations. The cultures were imaged using IncuCyte ZOOM for 48 h (MDA-MB-231(SA) cells) or 96 h (MFM223 cells). The relative wound closure (mean ± SD, n = 8) is presented at the indicated time points. (b) MDA-MB-231(SA) cells were used in an invasion assay, where the cells were covered with collagen type I matrix. The cultures were imaged using IncuCyte ZOOM for 72 h. The relative invasion (mean ± SD, n = 8) is presented for each treatment group. Statistical significances were analyzed for the area under the curve (AUC) over time and are shown for the comparisons to the control groups (* p < 0.05, ** p < 0.01, *** p < 0.001). Note that the inhibitor concentrations used for MDA-MB-231(SA) and MFM223 cells are different