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. 2021 Feb 25;11:4615. doi: 10.1038/s41598-021-84222-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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V1G1 modulates invasive phenotype of breast cancer cells. V1G1-depleted MCF7 (V1G1i) (a) and HA-V1G1 overexpressing MDA-MB-231 (b) or Hs578T cells (c) were imaged during wound healing assay at initial time point (T0) and 8 h after the scratch. Cell migration was measured as the ratio between the closed area of the wound in control cells (HA or scr) and HA-V1G1 overexpressing or V1G1 silenced cells. Scale bar = 100 µm. (d) Gelatin zymography was performed using conditioned medium of MDA-MB-231 cells overexpressing HA-V1G1 and control. Representative results are shown. (e) Western blot analysis was performed using specific anti-MMP-2, anti-HA and anti-tubulin antibodies. Intensity of bands was measured using the software Image Lab. Data represent the mean ± s.e.m. of at least three experiments. Statistical analysis was performed using Student’s t-test with control cells (scr or HA) as referring sample set to 1. **p ≤ 0.01; ***p ≤ 0.001.