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. 2021 Feb 25;11:4615. doi: 10.1038/s41598-021-84222-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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V1G1 overexpression affects RAC1 signaling. (a) MDA-MB-231 cells overexpressing HA-V1G1 and control (HA) were lysed and subjected to western blot analysis using anti-HA, anti-RAC1, anti-cofilin and anti-tubulin antibody. Quantification of RAC1 and cofilin protein amount is shown. Data represent the mean ± s.e.m. of at least three experiments. **p ≤ 0.01; i***p ≤ 0.001. (b) HA-V1G1 overexpressing MDA-MB-231 cells were immunolabeled with anti-HA followed by Alexa488 conjugated secondary antibody to discriminate between cells overexpressing HA-V1G1 and control cells, anti-vinculin followed by Alexa568 conjugated secondary antibody while nuclei were stained with DAPI. Bars = 10 µm.