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. 2021 Feb 25;11:4615. doi: 10.1038/s41598-021-84222-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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V1G1 modulates late endocytic compartments in MCF7 cells. (a) V1G1-depleted MCF7 cells (V1G1i) were labeled with Lysotracker Red and analyzed by confocal microscopy, scale bar: 10 µm. (b) The size of organelles was quantified by ImageJ software. Data represent the mean ± s.e.m. (at least 4 independent experiments, ≥ 15 cells /sample for each experiment) ***p ≤ 0.001. (c) Lysotracker Red intensity was quantified by ImageJ software and Corrected Total Cell Fluorescence was calculated. Data represent the mean ± s.e.m. (at least 4 independent experiments, ≥ 15 cells/sample for each experiment) *p ≤ 0.01. (d) Relative protein abundance of the two active isoforms of TFE3 and V1G1 was assessed by western blotting, quantified by densitometry and normalizing against tubulin. Data represent the mean ± s.e.m of at least three independent experiments (*p ≤ 0.01, **p ≤ 0.01, ***p ≤ 0.001).