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. 2021 Feb 25;11:4584. doi: 10.1038/s41598-021-83862-1

Table 1.

Distribution of tfs3 and tfs4 ICE in DU, NCGC, and CG patients.

Type of T4SS DU (%), n = 46 NCGC (%), n = 51 CG (%), n = 39 Total (%), n = 136
tfs negative 6 (13.0) 1 (0.2) 6 (15.3) 13 (9.6)
tfs positive 40 (87.0) 50 (98.0) 33 (84.6) 123 (90.4)
Incomplete tfs | Complete tfs* 21 (45.6) 19 (41.3) 35 (68.6) 15 (29.4) 19 (48.7) 14 (35.9) 75 (55.1) 48 (35.3)
Single tfs
tfs3 2 (4.3) 7 (15.2) 2 (3.9) 3 (5.8) 4 (10.2) 0 8 (5.8) 10 (7.3)
tfs4 13 (28.2) 4 (8.7) 20 (39.2) 9 (17.6) 8 (20.5) 7 (17.9) 41 (30.1) 20 (14.7)
Double tfs**
Incomplete tfs3 and tfs4 | Complete tfs3/tfs4 6 (13.0) 8 (17.4) 13 (25.5) 3 (5.8) 7 (17.9) 7 (17.9) 26 (19.1) 18 (13.2)
tfs4 module
L1 10 (21.0) 18 (35.3) 13 (33.3) 41 (30.1)
L2 21 (45.6) 27 (52.9) 16 (41.0) 64 (47.0)
C1 9 (19.5) 16 (31.3) 15 (38.4) 40 (29.4)
C2 2 (4.3) 0 2 (5.1) 4 (2.9)
R1 4 (8.7) 13 (25.5)a 10 (25.6)b 27 (19.8)
R2 14 (30.4) 14 (27.4) 10 (25.6) 38 (27.9)
Other T4SS
cagPAI 45 (97.8) 51 (100) 34 (87.1) 130 (96.1)
comB*** 46 (100) 51 (100) 39 (100) 136 (100)

80% < Identity of gene only counted.

Complete tfs harbored 11 vir genes (virB2, virB3, virB4, virB6, virB7, virB8, virB9, virB10, virB11, virD4, and virD2)

** Double tfs were divided into: both of complete tfs, one of complete tfs, and both of tfs incomplete form cagPAI harbored all ten vir genes (virB2, virB3, virB4, virB6, virB7, virB8, virB9, virB10, virB11, and virD4).

***comB harbored all seven vir genes (virB2, virB3, virB4, virB7, virB8, virB9, and virB10).

aIndicated the significant different between NCGC to DU at P < 0.05.

bIndicated the significant different between CG to DU at P < 0.05.