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. 2021 Feb 25;11:4549. doi: 10.1038/s41598-021-83952-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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T cell migration is enhanced in the presence of SPARC in vitro. dsRed⁺ T cells were seeded in matrigel in the presence or absence of SPARC and the presence or absence of recombinant CCL21. Cell migration was imaged over 20 min in response to no chemokine, a constant concentration of chemokine, or a chemokine gradient. Videos of cell migration were analyzed. (a) Tracks of T cells migrating in response to media alone, a constant chemokine field, or in response to a gradient of CCL21 were normalized to begin at the origin to visualize migration trends. (b) Cell velocity, displacement, and meandering index were quantified using Volocity software and migration data was analyzed using the Mann–Whitney test. Data are representative of at least 2 individual experiments and are represented as mean ± SEM. Thick black lines indicate comparisons + / − SPARC, thin grey lines indicate comparison + / − chemokine. To simplify, only significant comparisons are annotated *p < 0.05, ***p < 0.001.