Figure 2.

MPSSS Reduces CRC metastasis in a mouse model. CT-26-GFP cells were injected subcutaneously into the footpads of BALB/c mice. Draining lymph nodes different groups were harvested at the same time after cervical dislocation. Draining lymph nodes were taken photos by Caliper IVIS Lumina II under bright field (A) and fluorescent field (B, C). Fluorescence intensity was quantified and analyzed (D). Then, all lymph nodes were ground separately and digested with collagenase. Flow cytometry analysis was shown to display the percentage of GFP (+) cells migrating to draining lymph nodes (E, F). Data were analyzed by t-test. (*p < 0.05. **p < 0.01).