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. 2021 Feb 12;10:547683. doi: 10.3389/fonc.2020.547683

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Copyright © 2021 Wang, Zeng, Zhu, Wan, Lei, Yao, Duan, Zhang, Cheng, Tao and Qin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of MPSSS on the migration of SVEC4-10. 30% CAF supernatant treated with MPSSS (30 µg/ml) was used to culture SVEC4-10 cells. Photos were taken with a camera under a microscope at different time points (0 and 8 h) (A, B). Thirty percent CAF supernatant treated with and without MPSSS (30 µg/ml) was added to the bottom chamber (C, D). Gap area and cell number were expressed as the mean ± SD from triplicate measurements and analyzed by one-way ANOVA and t-test. (**p < 0.01. ***p < 0.001).