Table 1.
AfAgo-DNA complexes observed by AFM (see Supplementary Fig. S4).
| Protein | Complex | ||
|---|---|---|---|
| DNA loops, % | Linear | ||
| Protein bound to one end, % | Protein bound to both ends, % | ||
| WT AfAgo | 51% (n = 95) | 35% (n = 66) | 13% (n = 26) |
|
Monomer (n = 20)a Dimer (n = 47) Other (n = 28) |
Monomer (n = 33) Dimer (n = 22) Other (n = 11) |
Monomer–monomer (n = 6) Dimer-dimer (n = 4) Monomer–dimer (n = 13) Other (n = 3) |
|
| AfAgoΔ | 26% (n = 44) | 40% (n = 67) | 34% (n = 58) |
|
Monomer (n = 24) Dimer (n = 12) Other (n = 8) |
Monomer (n = 38) Dimer (n = 24) Other (n = 5) |
Monomer–monomer (n = 20) Dimer-dimer (n = 8) Monomer–dimer (n = 25) Other (n = 5) |
|
aThe calculated volume of WT AfAgo (50.8 kDa) and AfAgoΔ (49.9 kDa) proteins is approx. 100 nm3. Thus, the measured protein volume data from AFM images was divided into three populations by their theoretical volume: monomer (< 150 nm3), dimer (150–250 nm3), and other (> 250 nm3). For details see Supplementary Data and Figure S4.