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. 2021 Feb 25;11:4555. doi: 10.1038/s41598-021-83465-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Kdr genotyping in the Na_V IIIS6 segment in Aedes aegypti from Lahore, Pakistan. (a) The high-resolution melting analyses (HRM) difference plot, where each line represents a sample, is grouped into three variants. Sequencing of some samples of each variant, summed with TaqMan genotyping of the F1534C SNP indicated their genotypes, which then rendered the genotypic (b) and allelic frequencies (c), considering the 1520 and 1534 sites.