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. 2021 Feb 25;11:4555. doi: 10.1038/s41598-021-83465-w

Table 3.

Primers and probes for TaqMan SNP genotyping qPCR assays for kdr mutations in Aedes aegypti.

NaV Site, segment Assay IDa Variation Primers Probes
410, IS6 AN2XA9W

GTA/TTA

(Val/Leu)

for: GTGGCACATGCTCTTCTTCATT

rev: GGCGACAATGGCCAAGATC

Val: VIC-TCGTTCTACCTTGTAAATT-NFQ

Leu: FAM-TTCGTTCTACCTTTTAAATT-NFQ
989, IIS6 AH21C3C

TCT/CCT

(Ser/Pro)

for: TGATCGTGTTCCGGGTATTATGC

rev: CCATCACTACGGTGGCCAAAA

Ser: VIC-CCCACATGGATTCGAT-NFQ

Pro: FAM- CCACATGGGTTCGAT-NFQ
1016, IIS6 AHX1KC9

GTT/ GGT

(Val/Gly)

for: CGTGCTAACCGACAAATTGTTTCC

rev: TTGGACAAAAGCAAGGCT

Val: VIC-AGAAAAGGTTAAGTACCTGTGCG-NFQ

Gly: FAM- AGAAAAGGTTAAGTACCTGTGCG-NFQ
1534, IIIS6 AHWSL61

TTC/TGC  

(Phe/Cys)

for: TCGCGAGACCAACATCTACATG

rev: GATGATGACACCGATGAACAGATTC

Phe: VIC-AACGACCCGAAGATGA-NFQ

Cys: FAM-ACGACCCGACGATGA-NFQ

a Identification of the customized TaqMan SNP Genotyping Assay (Thermo Fischer).