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. 2021 Feb 10;23:1288–1303. doi: 10.1016/j.omtn.2021.02.003

Figure 2.

Figure 2

Lnc-CTSLP4 overexpression inhibits GC cell EMT, migration and invasion in vitro

(A and B) qRT-PCR analysis of lnc-CTSLP4 expression in GC cells after shRNA knockdown (A) or overexpression (B) of lnc-CTSLP4. ∗p < 0.05, ∗∗p < 0.01. (C) Morphological change of MGC803 cells after overexpression of CTSLP4 (100×). (D) PCR-array analysis of EMT-associated genes in MGC803-AV-CTSLP4 and MGC803-AV-CON cells. (E) qRT-PCR analysis of E-cadherin, N-cadherin, and Snail mRNA in GC cells after overexpression or shRNA knockdown of lnc-CTSLP4. ∗∗p < 0.01, ∗∗∗p < 0.001. (F) Western blot analysis of E-cadherin, N-cadherin, and Snail protein in GC cells (MGC803, AGS, HGC27, MKN28 cells) after knockdown of lnc-CTSLP4 (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (G and H) Representative IHC imaging of E-cadherin and N-cadherin (magnification: 25× and 100×). (I) Correlation of lnc-CTSLP4 expression (RNA-FISH) and EMT makers (E-cadherin and N-cadherin, IHC) in GC (GC cohort 2, ∗p < 0.05, ∗∗p < 0.01). (J) The migration and invasion abilities of GC cells after overexpression or knockdown of lnc-CTSLP4 (Transwell assay, ∗∗p < 0.01, ∗∗∗p < 0.001, 200×). (K and L) Wound-healing assays of GC cells after transfection with AV-CTSLP4 or sh-CTSLP4 (∗∗p < 0.01, ∗∗∗p < 0.001, magnification: 200×).