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. 2021 Feb 25;11:4709. doi: 10.1038/s41598-021-84180-2

Figure 2.

Figure 2

Flies preferentially consumed endocannabinoids and synthetic cannabinoids. Flies were presented with food containing endocannabinoids and synthetic cannabinoids for four consecutive days. Flies preferentially consumed food with endocannabinoids, including 0.1 mg/ml AEA (A), 0.1 mg/ml 2-AG (B) and 0.1 mg/ml AA (n = 18–21). However, a significant preference for 0.1 mg/ml 2-LG was detected only on day 3 (C). (D) A selective preference for 0.5 mM CB1/2 receptors agonist CP55940, but not WIN55212-2, was detected for four days (n = 18–20). (E) Flies did not consistently display significant preference for 0.5 mM CB1/2 receptors antagonists AM251 or AM630 (n = 18–24). (F) Flies displayed a strong preference for 0.5 mM PF3845 over the entire feeding course (n = 21). Data are mean ± S.E.M. One-sample t test was applied to determine statistical significance. *p < 0.05, **p < 0.01, and ***p < 0.001.