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. 2021 Feb 25;11:4653. doi: 10.1038/s41598-021-84067-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A circular marker was set surrounding the optic nerve head (ONH) to measure the mean blur rate (MBR) (A). The “vessel extraction’’ function of the software then identified the vessel and tissue areas on the ONH so that the MBR could be assessed separately as the vessel areas (MBR-vessel) and the tissue areas (MBR-tissue) (B). The rectangular areas (1 to 19) were set semi-automatically to the vessels around the optic nerve head concentrically and the total retinal flow index (TRFI) was calculated (C).