Table 3.
Overview of parameters applied in the electrofusion of experiment 4.
| Fusion | Acceptor | Donor | Acceptor : donor ratio | Electrofusion | Regeneration | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cell line | IOAz | Cultivar/accession | UVCz | ACy | DCx | Acceptor | Fusion | ||
| A | Celeriac ‘Diamant' 23 | 5 | Carrot ‘Dolanka' | 3 | 1: 1 | 3 V; 40 s | 25 V; 60 µs; 1 | Died | Callus |
| B | Celeriac ‘Diamant' 23 | 10 | Carrot ‘Dolanka' | 3 | 1: 1 | 3 V; 40 s | 25 V; 60 µs; 1 | Died | Died |
| C | Celeriac ‘Diamant' 23 | 20 | Carrot ‘Dolanka’ | 3 | 1 : 1 | 3 V; 40 s | 25 V; 60 µs; 1 | Died | Died |
| D | Celeriac ‘Diamant' 24 | 20 | Carrot ‘Dolanka' | 6 | 1 : 1 | 3 V; 40 s | 25 V; 60 µs; 1 | Shoots | Shoots |
| E | Celeriac ‘Diamant' 24 | 20 | Carrot ‘Dolanka' | 6 | 1 : 2 | 3 V; 40 s | 25 V; 60 µs; 1 | Died | Died |
| F | Celeriac ‘Diamant' 24 | 25 | Carrot ‘Parmex' | 6 | 1 : 1 | 2.5 V; 40 s | 25 V; 60 µs; 2 | Shoots | Shoots |
| G | Celeriac ‘Diamant' 24 | 25 | Carrot ‘Parmex' | 6 | 1: 2 | 2.5 V; 40 s | 25 V; 60 µs; 2 | Shoots | Shoots |
| H | Celeriac ‘Diamant' 24 | 25 | Coriander, csR1 | 6 | 1 : 1 | 3 V; 40 s | 25 V; 60 µs; 2 | Died | Died |
| I | Celeriac ‘Diamant' 24 | 30 | Carrot ‘Parmex' | 6 | 1 : 2 | 3 V; 40 s | 25 V; 60 µs; 1 | Died | Died |
| J | Celeriac ‘Diamant' 25 | 20 | Carrot ‘Karotan’ | 6 | 1 : 2 | 3 V; 40 s | 25 V; 60 µs; 1 | Died | Died |
| K | Celery ‘Daybreak' 4.1 | 20 | Carrot ‘Dolanka' | 2 | 1 : 1 | 3 V; 40 s | 25 V; 60 µs; 2 | Died | Died |
| L | Celery ‘Daybreak' 4.1 | 20 | Carrot ‘Dolanka' | 2 | 1 : 1 | 3 V; 40 s | 50 V; 60 µs; 2 | Died | Died |
Per electrofusion the regeneration status of the IOA control and the fusion samples, 16 weeks after the start of the culture, is shown.
zExposure time (min).
yAlternating current: alignment voltage; alignment duration.
xDirect current: pulse voltage; pulse duration; number of pulses.