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. 2021 Feb 25;11:4641. doi: 10.1038/s41598-021-84169-x

Table 2.

LB hens—weeks 16 to 24: differential metabolite concentrations between weeks 16 and 24 in plasma according to FDR adjusted P-value from t-student’s test.

Metabolite (µmol/l) Week 16 Week 24 FDR adjusted p-value t-valuedf
Amino acids
Tyrosine 337 ± 9.9 172 ± 7.3  < 0.001 13.0918
Threonine 557 ± 21.2 313 ± 20.1  < 0.001 7.7018
Tryptophan 144 ± 6.4 91.8 ± 4.74  < 0.001 6.7718
Histidine 262 ± 10.2 162 ± 16  < 0.001 5.4118
Asparagine 319 ± 14.1 210 ± 15  < 0.001 5.0418
Arginine 503 ± 10.7 389 ± 22.1  < 0.001 4.5618
Glutamine 1297 ± 35.7 883 ± 95.8  < 0.01 3.9818
Alanine 1156 ± 46.6 813 ± 63.7  < 0.01 3.9618
Lysine 474 ± 31.7 310 ± 38.5  < 0.01 3.9618
Proline 763 ± 29.2 592 ± 35.6  < 0.01 3.5818
Biogenic amines
Carnosine 20.3 ± 1.4 7.5 ± 0.32  < 0.001 12.7618
trans-4-OH-Pro 134.4 ± 7.9 30.8 ± 3.6  < 0.001 11.4418
Alpha-AAA 2.1 ± 0.18 1.04 ± 0.1  < 0.001 5.3518
Sarcosine 21.6 ± 0.8 12 ± 1.57  < 0.001 5.3218
Spermine 0.58 ± 0.06 0.30 ± 0.01  < 0.001 4.9918
Phospholipids
Sum of PCs 1862 ± 95.8 4728 ± 455  < 0.001  − 8.5618
Sum of lysoPCs 54.4 ± 3.38 19.3 ± 1.11  < 0.001 10.9118
Sum of SMs 237.4 ± 11.3 160.5 ± 14.2  < 0.01 4.1518

Comparison between LB hens at 16 (n = 10) and 24 (n = 10) weeks of age. Values are showed as means ± SEM.

ADMA asymmetric dimethylarginine, Alpha-AAA alpha-aminoadipic acid, Met-SO methionine sulfoxide, PC glycerophosphatidylcholines, SM sphingomyelins.

FDR-adjusted p < 0.05 as significance level was used. t-value indicates the ratio between the difference between and within both groups. Degrees of freedom (df) are shown in the figure as subscript of t-values.