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. 2021 Feb 25;11:4641. doi: 10.1038/s41598-021-84169-x

Table 3.

LSL hens—week 16 to 24: differential metabolite concentrations in plasma according to FDR adjusted p-value from t-student’s test.

Metabolite (µmol/l) Week 16 Week 24 FDR adjusted p-value t-valuedf
Amino acids
Threonine 548 ± 21.6 275 ± 24.4  < 0.001 7.7118
Tryptophan 168 ± 6.3 89.3 ± 7.5  < 0.001 7.5818
Asparagine 347 ± 18.4 200 ± 12  < 0.001 7.1118
Histidine 260 ± 11.4 153 ± 10.5  < 0.001 6.5818
Glycine 1050 ± 32.3 689 ± 44.6  < 0.001 6.5418
Alanine 1090 ± 47.2 680 ± 44.7  < 0.001 6.2918
Tyrosine 289 ± 13.4 183 ± 13.1  < 0.001 5.7318
Proline 898 ± 50.4 536 ± 48.4  < 0.001 5.3418
Arginine 546 ± 18.1 408 ± 20.1  < 0.001 5.1618
Aspartate 72 ± 5.7 45 ± 3.5  < 0.001 4.4418
Serine 1071 ± 29.1 868 ± 42.4  < 0.01 3.9618
Valine 516 ± 21.7 348 ± 37.4  < 0.01 3.9618
Methionine 230 ± 12 165 ± 12.3  < 0.01 3.5918
Leucine 664 ± 29.3 516 ± 37.2  < 0.01 3.4018
Glutamine 1294 ± 42.7 1057 ± 65.8  < 0.01 3.2618
Isoleucine 291 ± 11.7 228 ± 20.0  < 0.05 3.0318
Biogenic amines
trans-4-OH-Pro 134 ± 6.4 25 ± 1.79  < 0.001 20.4818
Sarcosine 24 ± 1.3 7.9 ± 0.74  < 0.001 10.9218
Carnosine 26.7 ± 1.2 10.2 ± 0.85  < 0.001 10.5318
ADMA 1.14 ± 0.05 0.75 ± 0.03  < 0.001 6.3418
Alpha-AAA 2.4 ± 0.2 1.2 ± 0.1  < 0.001 5.3318
Spermine 0.62 ± 0.05 0.36 ± 0.04  < 0.001 4.5418
Met-SO 19.9 ± 1 13.47 ± 1.32  < 0.01 4.0718
Kynurenine 0.44 ± 0.07 0.18 ± 0.02  < 0.01 3.7318
Taurine 774 ± 116 380 ± 49  < 0.01 3.1718
Putrescine 0.96 ± 0.1 1.69 ± 0.2  < 0.01  − 3.1518
Creatinine 2.95 ± 0.17 2.34 ± 0.2  < 0.05 2.4118
Phospholipids
Sum of PCs 1951 ± 65.7 4726 ± 393  < 0.001  − 9.4418
Sum of lysoPCs 60.5 ± 4 19.9 ± 1  < 0.001 13.7218
Sum of SMs 246 ± 12.2 161 ± 12  < 0.001 4.8818
Hexoses
Sum of hexoses 21,253 ± 998 17,995 ± 857  < 0.05 2.5318

Comparison between LSL hens at 16 (n = 10) and 24 (n = 10) weeks of age. Values are showed as means ± SEM.

ADMA asymmetric dimethylarginine, Alpha-AAA alpha-aminoadipic acid, Met-SO methionine sulfoxide, PC glycerophosphatidylcholines, SM sphingomyelins.

FDR-adjusted p < 0.05 as significance level was used. t-value indicates the ratio between the difference between and within both groups. Degrees of freedom (df) are shown in the figure as subscript of t-values.