TABLE 2.

Primers for SYN8 deletion and reintegration.

Primers	Primer sequence (5′→ 3′)
pSyn8F	TTGCGTTTGAACCCCGGGATTAAGGATTGGATTACTGTAT ACAAGCAGCGAAGGAGGGCCGCTGATCACG
pSyn8R	GAAATATTGAATGGTAGTATCTGTAATAATGGTTAATCTCAT CAAACTGAGATGCTCATCGTGAGGCTGG
pHIScheckF	CAGCCATTGTATGCCACTGTATGA
pHIScheckR	CAGCTTTATCTCAGAAAACCAG
pSyn8checkF	GGATCTGAAGGTTTCATATGAGCTTGA
pSyn8checkR	GAAAAGTCATTGGGGCTGATGCA
pSyn8compF	AATCTAGAATTCTATCCTCCATGGGCTCCTT
pSyn8compR	AATCTAGATCTGTGATGAGCCGAATCAGAATC
pChr606 F1	AAGAATGCCAACCAAGGATTCACAATAATCCGAAGC
pChr606 R1	TTAGGCAAAGCATTTGTAAACCATTACAAGCACTC
pZeoORFcheckF1	AAGTTGACCAGTGCCGTTCCGGTG
pchr606check	CTAATGGGGATATAGAAAGATAGGG