FIGURE 4.

Effect of TLR-9 deficiency on leukocytes infiltration and inflammation related cytokines in the kidney following renal IRI. Inflammatory macrophage infiltration in WT and KO kidneys at 1 and 28 days following IRI were determined using flow cytometry. (A) Representative dot plots of CD45+ cells and different types of leukocytes (T cells and macrophages) of WT and KO mice at various time points following reperfusion. (B) Quantification of related immune cell infiltration, including CD 45+ cells, CD3+ T cells, F4/80^high, and F4/80^low subset in CD11b+. (C) Heatmap of mRNA expressions related to inflammation in renal 28 days post IRI. Data are shown as the mean ± SD or percentage and analyzed using the Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001. NS, no significant difference; IRI, ischemia-reperfusion injury; WT, wild-type; KO, knockout.