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. 2021 Feb 12;9:616610. doi: 10.3389/fcell.2021.616610

FIGURE 2.

FIGURE 2

Effects of TGF-β2 and BMP9 on SNAIL and SLUG expression. (A) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Snail mRNA expression after 3, 6, and 24 h treatments in MS-1 cells. (B) Western blot analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on SNAIL and SLUG protein expression after 6 and 24 h treatments in MS-1 cells. (C) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Slug mRNA expression after 3, 6, and 24 h treatments in MS-1 cells. (D) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Snail mRNA expression after 3 days treatments in MS-1 cells. (E) Western blot analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on SNAIL protein expression after 3 days treatments in MS-1 cells. (F) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Snail mRNA expression after 3, 6, and 24 h treatments in 2H11 cells. (G) Western blot analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on SNAIL and SLUG proteins expression after 6 and 24 h treatments in 2H11 cells. (H) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Slug mRNA expression after 3, 6, and 24 h treatments in 2H11 cells. (I) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Snail mRNA expression after 3 days treatments in 2H11 cells. (J) Western blot analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on SNAIL and SLUG proteins expression after 3 days treatments in 2H11 cells. (K) RT-qPCR analysis of the effects of TGF-β2 (1 ng ml–1) and BMP9 (5 ng ml–1) on Slug mRNA expression after 3 days treatments in 2H11 cells. All the mRNA expression levels were normalized to those of the housekeeping gene Gapdh. Results are expressed as mean ± SD. Representative results from three independent experiments are shown.