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. 2021 Feb 12;9:607341. doi: 10.3389/fcell.2021.607341

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Copyright © 2021 Zeng, Li, Zou, Chen, Li, Li, Ge, Chen and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The cell differentiation of hERO-NRs with 100 μg/ml PM2.5 exposure. (A–D) Immunostaining analysis of retinal differentiation-related markers in the hERO-NRs (after 3 weeks, 100 μg/ml PM2.5 treatment). (A,B) Co-tagging of Chx10 and HuCD at control group and PM2.5-100 μg/ml group. (C,D) Co-tagging of Chx10 and BRN3 in the control group and PM2.5-100 μg/ml group. (A1–D1) shows (A–D) without DAPI, respectively. (E,F) Immunostaining analysis of CRX and BRN3 in the hERO-NRs (after 3 weeks, 100 μg/ml PM2.5 treatment). (E1,F1) show (E,F) without DAPI, respectively. Scale bar = 50 μm.