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. 2021 Feb 12;11:626431. doi: 10.3389/fimmu.2020.626431

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Copyright © 2021 Cao, Khanal, Wang, Li, Zhao, Nguyen, Nguyen, Dang, Schank, Thakuri, Zhang, Lu, Wu, Morrison, El Gazzar, Ning, Moorman and Yao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HIV induces CD4 T cell death in productively infected and bystander cells during early infection via different mechanisms. (A) Representative dot plots (day 7) and summary of the percentage (%) of Av and 7AAD levels in HIV-infected and uninfected CD4 T cells, as determined by flow cytometry. (B, C) Representative dot plots and summary data showing active caspase-3% in productively HIV-infected (p24⁺) and bystander (p24^-) or uninfected cells at days 3, 5, and 7 in cultures. (D, E) Representative dot plots and summary data of active caspase-1% in HIV-infected (p24⁺) and bystander (p24^-) or uninfected cells at day 3, day 5, and day 7. (F–H) Representative dot plots and summary data of GPX4% in HIV-infected (p24⁺) and bystander (p24^-) or uninfected cells at days 3, 5, and 7.