FIGURE 3.

Combination treatment normalized gut microbiota of aGVHD mice. (A) Shannon index of alpha diversity was examined by 16S high-throughput sequencing on day 7 in the GVHD, control, and different treatment groups. n = 4–6/group. Statistical significances are indicated: The Wilcoxon rank-sum test. The top and bottom boundaries of each box indicate the 75th and 25th quartile values, respectively, and lines within each box represent the 50th quartile (median) values. Ends of whiskers mark the lowest and highest diversity values in each instance. (B) Simpson index of alpha diversity was examined by 16S high-throughput sequencing on day 8 in GVHD, control, and different treatment groups. n = 4–6/group. (C) Weighted UniFrac-based principal coordinates analysis (PCoA). The principal component was used to measure the shift of the intestinal bacterial composition structure in different groups of mice after BM transplantation at day 8. n = 4–6/group. BT, samples from mice without BM transplantation; ATCON, samples from BALB/c mice transplanted with BM from C57 donors only; ATGVHD, samples from BALB/c mice transplanted with BM and splenocytes from C57 donors; ATCSA, samples from BALB/c mice transplanted with BM and splenocytes from C57 donors treated with 5 mg/kg CsA; AT0.2X, samples from BALB/c mice transplanted with BM and splenocytes from C57 donors treated with 0.2 mL/kg XBJ; ATCOM, samples from BALB/c mice transplanted with BM and splenocytes from C57 donors treated with 2.5 mg/kg CsA and 0.5 mL/kg XBJ. ^*,**Compared with aGVHD group.