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. 2021 Feb 12;11:608279. doi: 10.3389/fphys.2020.608279

FIGURE 8.

FIGURE 8

XBJ maintained the integrity of the gut microenvironment and prevented doxorubicin (DOX)-induced cell death in Caco-2 cells. (A) Gut permeability assay to determine the influence of the combination therapy on the integrity of the intestine tissue of aGVHD mice. FITC–dextran concentrations (% of aGVHD) in serum were measured on day 7 after the transplant. Data are means ± SD. Data were analyzed using ordinary one-way analysis of variance for multiple comparisons. *Control compared with aGVHD, ***P < 0.001; #treatment groups compared with aGVHD; #P < 0.05, ##P < 0.01, ###P < 0.001. (B,C) The CCK-8 assay was used to determine the survival of Caco-2 cells in the presence of doxorubicin and XBJ. (B) Caco-2 cells were pretreated with XBJ as indicated for 24 h and were then treated with DOX for 12 h before subjecting to CCK-8 assay. (C) Caco-2 cells were treated with DOX for 12 h, and XBJ was added to the indicated groups for 24 h before subjecting to CCK-8 assay. Each experiment was repeated at least three times. *Control vs. DOX-treated groups; ***P < 0.001; #XBJ-treated groups vs. DOX-treated group; #P < 0.05, ##P < 0.01, ###P < 0.001.