Figure 7.

Bacillus ce. induces in vitro proliferation and IFN-γ expression by CD8⁺ T cells derived from recipient mice that were primed with BALB/c skin. FACS-sorted CD8+ T cells derived from naïve or C57BL/6 recipients transplanted with BALB/c or C3H/HeJ skin 4 weeks earlier were stained with CFSE and cultured in the presence of inactivated Bacillus ce. plus inactivated self APCs obtained from C57BL/6 splenocytes depleted of CD3+ T cells via magnetic micro-beads detachment. Twenty-four hours later, CD8+ T cell division was measured via FACS analysis after gating on CD8+ population (A), while one representative of three separate sets of histograms is shown. Besides, IFNγ expression by the lyzed cells was also measured via ELISA (B). Data of individual values are presented as median ± interquartile range (*P < 0.05, n = 4 mice/group).