TABLE 1.

Advantages and disadvantages of MSC functionalization in 3D spheroids.

Advantages	Disadvantages
• Increased stability of MSC immunophenotypic and molecular profiles • Enhanced stemness features and differentiation potential • Upon infusion, enhanced survival and homing in vivo • Enhanced secretory profile exerting mitogenic, anti-apoptotic, angiogenic, anti-fibrotic and anti-inflammatory properties	• Size variability depending on the technique used to generate spheroids •Depending on spheroid size, nutrients, oxygen, and waste concentration gradients within the spheroids •Depending on spheroid size, necrotic spheroid core formation •Depending on the clinical needs, development of reproducible, simple and cost-effective techniques are needed for large-scale production of MSC spheroids