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. 2021 Feb 12;11:603270. doi: 10.3389/fimmu.2020.603270

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Copyright © 2021 Jia, Jiang, Wang, Wang, Liu, Lv, Song, Li, Wang and Song

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry, immunofluorescence and flow cytometry analysis of the localization of CgDM9CP-4 protein. (A) Distribution of CgDM9CP-4 was visualized by Alexa Fluor 488-labeled goat-anti-rat antibody (upper panel), the rat pre-immuned serum was used as control (lower panel), the tissues were stained with Evans blue dye (red). Bar= 50 μm. (B) Expression of CgDM9CP-4 on the membrane of hemocytes as detected by flow cytometry. (C) Localization of CgDM9CP-4 in hemocytes. Nucleus was stained with DAPI (blue), CgDM9CP-4 (green) and the cell membrane were stained with CM-DIL (red). Bar= 10 μm. (D) Distinct gating by forward scatter (FSC) and Alexa Fluor 488-labeling and (left), contour by FSC and Alexa Fluor 488-labeling showing the specific group of CgDM9CP-4-positive hemocytes (middle), size and granularity of CgDM9CP-4-positive hemocytes were analyzed by Side scatter (SSC) and FSC (right).