FIGURE 5.

Histological changes showing restoration of the small intestine and colon and gut barrier dysfunction by the combination of SB and SJ in SHR. (A) HE staining of the small intestine. The representative images were taken from the gut tissue slides (Magnification, 10×). (B) HE staining of colon tissue (Magnification, 10×). (C) Alcian blue staining of goblet cells. (D) IHC staining of occludin in colon tissue; the dark brown staining indicates a positive stain by occludin. (E) The immunofluorescence staining of occludin in colon tissue (Magnification, 20×). (F–I) Western blotting results of occludin, ZO-1, and their respective quantification in the colon. Data are presented as mean ± SD. ###p < 0.001. ##p < 0.01. #p < 0.05.