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. 2021 Feb 4;20:625–634. doi: 10.1016/j.omtm.2021.01.014

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Viral production with lack of Ad5/3-Δ24 contamination

(A) Representative images of A549 cells transfected with IL-15-expressing adenovirus and wild-type virus. Images were taken 9 days post-transfection at two different magnification, 10× and 40×. (B) Ad5/3-Δ24 contamination was checked by amplifying the gp19k region (Ad5/3-Δ24) or the CMV and poly(A) region (GOI). A simulation of what should be expected is presented and type of samples are annotated. Lane MW represents a 1 kb gene ruler from Thermo Fisher. Lanes 4 and 9 represent Ad5/3-Δ24 virus. Lanes 1 and 6 represent CXCL9-expressing adenovirus. Lanes 2 and 7 represent CXCL10-expressing adenovirus. Lanes 3 and 8 represent IL-15-expressing adenovirus. (C) The actual representation of the wild-type contamination PCR assay. Lane 1 represents a 1 kb gene ruler from Thermo Fisher. Lanes 5 and 9 represent the unmodified Ad5/3-Δ24 virus. Lanes 2 and 6 represent CXCL9-expressing adenovirus. Lanes 3 and 7 represent CXCL10-expressing adenovirus. Lanes 4 and 8 represent IL-15-expressing adenovirus.