Figure 7.

Functional activity of chemokine-expressing viruses

(A) Chemokines from CXCL9, CXCL10, and IL-15-expressing viruses were measured using an ELISA. A549 cells were infected with 100 MOI of the viruses indicated, and at day 2 post-infection the supernatant was collected and tested. (B) Migration was assessed using a Transwell system. Chemokine-expressing viruses were added to MDA-MB-436 cells at MOI 100 at the lower chamber. To the top chamber, calcein green-labeled PBMCs were added, and at day 2 the number of cells at the bottom (migrated cells) were counted manually. (C) Visual representation of migrated green labeled PBMCs. Scale bar, 400 nm.