REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Neurofascin (extracellular domain)	NeuroMab/Antibodies Incorporated	Cat# 75–172; RRID: AB_2282826
Rabbit polyclonal anti-TRIM46	Synaptic Systems	Cat. # 377 003; RRID: AB_2631232
Mouse monoclonal anti-GM130	BD Bioscience	Cat. # 610823; RRID: AB_398142
Rabbit polyclonal anti-RTN4a (Nogo)	Novus Biologicals	Cat. # NB100-56681; RRID: AB_838641
Mouse monoclonal anti-ERK1/2 (p44/42 MAPK)	Cell Signaling	Cat. # 4696; RRID: AB_390780
Rabbit polyclona anti-phospho-ERK1/2 (phospho-Thr202/Tyr204)	Cell Signaling	Cat. # 9101; RRID: AB_331646
Mouse monoclonal anti-Rab6; Clone: 5B10	Prof. Angelika Barnekow (Münster University)	N/A
Rabbit polyclonal anti-myc	Santa Cruz	Cat. # SC-40; RRID: AB_2857941
Rabbit polyclonal anti-GFP	Abcam	Cat. # ab290; RRID: AB_303395
Goat Anti-Rabbit IgG Antibody, IRDye 800CW Conjugated	LI-COR Biosciences	Cat. # 827-08364; RRID: AB_10793856
Goat Anti-Mouse IgG Antibody, IRDye 680LT Conjugated	LI-COR Biosciences	Cat. # 827-11081; RRID: AB_10795015
anti-mouse Alexa647	Thermo Fisher	Cat. # A21236; RRID: AB_2535805
anti-rabbit Alexa647	Thermo Fisher	Cat. # A21245; RRID: AB_2535813
anti-mouse Alexa405	Thermo Fisher	Cat. # A-31553; RRID: AB_221604
anti-mouse Alexa488	Thermo Fisher	Cat. # A11029; RRID: AB_138404
anti-rabbit Alexa488	Thermo Fisher	Cat. # A11034; RRID: AB_2576217
anti-mouse Alexa568	Thermo Fisher	Cat. # A11031; RRID: AB_144696
anti-rabbit Alexa568	Thermo Fisher	Cat. # A11036; RRID: AB_10563566
Chemicals, Peptides, and Recombinant Proteins
Recombinant mouse Brain Derived Neurotrophic Factor (BDNF)	Sino Biological	50240-MNAS
Biotin	Sigma	B4639
Dynasore	Sigma	D7693
Rapalog (A/C Heterodimerizer)	Takara Bio	635056
Lipofectamine 2000	Thermo Fisher	11668019
Surface-SNAP-Alexa-488	NEB	S9129S
Surface-SNAP-Alexa-546	NEB	S9132S
Surface-SNAP-Alexa-647	NEB	S9136S
SYLGARD 184 Silicone Elastomer Kit	Dow	N/A
Experimental Models: Cell Lines
Human embryonic kidney 239T (HEK293T)	ATCC	CRL-3216
Experimental Models: Organisms/Strains
Rat (Wistar)	Janvier	N/A
Oligonucleotides
Rab27A (rat) shRNA targeting sequence: CCAGTGTACTGTACCAGTA	This study, based on (Arimura et al., 2009)	N/A
Rab27B (rat) shRNA targeting sequence: CAACATTTCTTTACCGATA	This study, based on (Arimura et al., 2009)	N/A
KIF1A (rat) shRNA targeting sequence: CGAATATGCTGACATCTTC	(Kevenaar et al., 2016)	N/A
KIF5C (rat) shRNA targeting sequence: TGAGATCACTTGGACAAA	(Pan et al., 2019)	N/A
Rab6A (rat) shRNA targeting sequence: CACCTATCAGGCAACAATT	(Schlager et al., 2010)	N/A
Rab6B (rat) shRNA targeting sequence: CACCTACCAGGCAACCATC	(Schlager et al., 2010)	N/A
Rab6A mutagenesis primer Q72L-Fw: ACAGCAGGTCTAGAGCGGTTC	This study	N/A
Rab6A mutagenesis primer Q72L-Rev: GTCCCATAATTGCAATCGTAC	This study	N/A
Rab6A mutagenesis primer T27N-Fw: GTTGGAAAGAACTCTTTGATC ACCAGATTCATGTATGACAG	This study	N/A
Rab6A mutagenesis primer T27N-Rev: GCTTTGCTCCCCCAGGAA	This study	N/A
Recombinant DNA
pGW1-CMV	(Hoogenraad et al., 2005)	N/A
pSuper vector	(Schlager et al., 2010)	N/A
pBio-GFP	Hoogenraad lab	N/A
pBirA	Hoogenraad lab	N/A
TrkB-GFP	Prof. Rosalind Segal (Harvard University)	N/A
SNAP-myc-TrkB	This study	N/A
RUSH-TrkB-GFP	This study	N/A
RUSH-SNAP-TrkB	This study	N/A
RUSH-mRFP-NPY	This study	N/A
GFP-Rab6A	Hoogenraad lab	N/A
mCherry-Rab6A	Hoogenraad lab	N/A
mCherry-Rab6A-Q72L	This study	N/A
mCherry-Rab6A-T27N	This study	N/A
mRuby3-Rab3C	This study	N/A
mRuby3-Rab27B	This study	N/A
mRFP-Rab11A	Hoogenraad lab	N/A
mRFP-Rab8A	Hoogenraad lab	N/A
mRFP-KIF5Cmd_(aa 1-559)-FKBP	Hoogenraad lab	N/A
FRB-myc-KIF5Atd_(aa 375-1032)	Hoogenraad lab	N/A
FRB-myc-KIF5Btd_(aa 374-963)	Hoogenraad lab	N/A
FRB-myc-KIF5Ctd_(aa 376-955)	Hoogenraad lab	N/A
FRB-myc-KIF17td_(aa 376-1028)	Hoogenraad lab	N/A
FRB-myc-KIF3Atd_(aa 386-702)	Hoogenraad lab	N/A
FRB-myc-KIF1Atd_(aa 395-1698)	Hoogenraad lab	N/A
FRB-myc-KIF1Bα_td_(aa 390-1153)	Hoogenraad lab	N/A
FRB-myc-KIF1Bβ_td_(aa 390-1770)	Hoogenraad lab	N/A
FRB-myc-KIF1Ctd_(aa 395-1103)	Hoogenraad lab	N/A
FRB-myc-KIF13Atd_(aa 397-1770)	Hoogenraad lab	N/A
FRB-myc-KIF21Atd_(aa 377-1661)	Hoogenraad lab	N/A
FRB-myc-KIF21Btd_(aa 410-1624)	Hoogenraad lab	N/A
Bio-KIF5Atd_(aa 375-1032)	This study	N/A
Bio-KIF5Btd_(aa 374-963)	This study	N/A
Bio-KIF5Ctd_(aa 376-955)	This study	N/A
Bio-KIF17td_(aa 376-1028)	This study	N/A
Bio-KIF3Atd_(aa 386-702)	This study	N/A
Bio-KIF1Atd_(aa 395-1698)	This study	N/A
Bio-KIF1Bβ_td_(aa 390-1770)	This study	N/A
Bio-KIF1Ctd_(aa 395-1103)	This study	N/A
Bio-KIF13Atd_(aa 397-1770)	This study	N/A
Bio-KIF21Atd_(aa 377-1661)	This study	N/A
Bio-KIF21Btd_(aa 410-1624)	This study	N/A
Software and Algorithms
Fiji/ImageJ	NIH	Imagej.net
Kymoreslicewide	Dr. Eugene Kathrukha (Utrecht University)	https://github.com/ekatrukha/KymoResliceWide
Prism 7.0	Graphpad	https://www.graphpad.com/scientific-software/prism/
R-Software	R Project	https://www.r-project.org/
Other
3-Compartment Microfluidic Device (MFC) mold	Prof. Frederic Saudou (Virlogeux et al., 2018)	N/A