Fig. 1.

Suppression of adipogenic differentiation by treatment with myonectin. (A) Effects of myonectin on cell viability. The viability of untreated control cells (Non) was defined as 100%. (B) Effects of myonectin on the differentiation of 3T3-L1 cells. Differentiation of con-fluent 3T3-L1 cells was induced by the MDI cocktail (0.5 mM IBMX, 1 μM Dexamethasone, and 10 μg/ml insulin). The 3T3-L1 cells were treated with different concentrations (0.05-2 μg/ml) of myonectin during differentiation. On day 10 of differentiation, the fully differentiated adipocytes were fixed and stained with oil red O solution for visualization of the lipid accumulation. (C-F) mRNA levels of adipogenic genes in mature 3T3-L1 adipocytes. Each gene was normalized to Rpl32. All quantitative data are the means ± SD (n = 6). *P < 0.05, **P < 0.005, ***P < 0.0005 compared to untreated control cells on day 0 (Day 0, Non).