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. 2021 Feb 7;24(3):102157. doi: 10.1016/j.isci.2021.102157

Figure 4.

Figure 4

Developmental pyramidal hyperexcitation produces enduring alterations in intrinsic excitability and synaptic E/I ratio of L5 prefrontal cortex pyramidal neurons

(A) Schematic of mouse prefrontal cortex brain slice featuring LMO3 pyramidal neurons.

(B) Firing response of biocytin-filled L5 prefrontal cortex pyramidal neurons to depolarizing current ramps (dotted line indicates threshold) in VEHP4-14 (blue) and CTZP4-14 (red) groups (scale bars: 100 μm). LMO3 expression confirmed with blue light stimulation.

(C) Example traces of firing response to depolarizing square current injections of increasing magnitude.

(D) Frequency-current relationship of L5 pyramidal neurons. Bars represent mean ± SEM.

(E) Summary graphs showing effect of developmental hyperexcitation on firing threshold, rheobase, maximum firing rate, and input resistance. Bars represent mean ± SEM.

(F) Example traces of mEPSCs recorded from LMO3 positive L5 pyramidal neurons (LMO3 expression confirmed with blue light stimulus at −70 mV, left).

(G) Summary graphs showing effect of developmental hyperexcitation on mEPSC frequency and amplitude. Bars represent mean ± SEM.

(H) Example traces of mIPSCs recorded from LMO3 positive L5 pyramidal neurons.

(I) Summary graphs showing effect of developmental hyperexcitation on mIPSC frequency and amplitude. Bars represent mean ± SEM.

(J) Summary graph showing effect of developmental hyperexcitation on E/I ratio of mPSC frequency, amplitude, and synaptic drive. Bars represent mean ± SEM. ∗p < 0.05, ∗∗pP < 0.01, ∗∗∗p < 0.001. See also Table S1.