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. 2021 Feb 23;8(1):e000861. doi: 10.1136/bmjresp-2020-000861

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Relationship between ion transporter activities and baseline epithelial properties. The effect of 48 hours treatment with normal (37°C) and low (29°C) temperature on transepithelial potential difference (TEPD) across non-cystic fibrosis (non-CF) and CF (F508del/F508del) primary nasal epithelial cells was analysed. Correlations between baseline TEPD and amiloride (100 μM)-sensitive ΔTEPD (A) or ΔTEPD from cystic fibrosis transmembrane conductance regulator (CFTR) activation (B) are presented. Values for CFTR activation represent total changes in response to VX-770 (1 µM) and Fsk/IBMX (20 µM/100 µM) application. (C–E) Absolute values for TEPD, short-circuit currents (Isc) and transepithelial electrical resistance (TEER) in non-CF epithelial cells at normal (37°C) and low (29°C) temperature after the acute addition of amiloride were quantified. Data in panels A and B are presented as individual replicates (n=12–34 replicates, from three individual non-CF and three individual CF donors) and analysed using linear regression. Data in panels C–E are presented as mean±SD. Brackets and asterisks indicate significant differences (t-test; **p<0.01).