Table 1.
Antibody mix for the extracellular staining of splenic T cells
| Fluorophore | Marker | Final dilution | Volume for 1 sample |
|---|---|---|---|
| eFluor 506 | Fixable viability dye | 1/200 | 0.5 μL |
| CD16/32 (mouse BD Fc block) | 1/100 | 1 μL | |
| PE-Cy5 | CD3e | 1/200 | 0.5 μL |
| APC-eFluor 780 | CD4 | 1/200 | 0.5 μL |
| PE-Cy7 | CD8a | 1/600 | 0.16 μL |
| BUV395 | CD25 | 1/100 | 1 μL |
| Alexa Fluor 700 | CD44 | 1/100 | 1 μL |
| PE | CD62L | 1/200 | 0.5 μL |
| BV421 | CD120b | 1/100 | 1 μL |
| Sum of antibodies | 6.16 μL | ||
| 1× D-PBS | 43.84 μL | ||
| BD Horizon Brilliant Stain Buffer | 1/2 | 50 μL | |
| Final staining volume | 100 μL | ||