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Antibody mix for the intracellular staining of splenic T cells
| Fluorophore | Marker | Final dilution | Volume for 1 sample |
|---|---|---|---|
| PE-eFluor 610 | CD152 | 1/50 | 2 μL |
| APC | FoxP3 | 1/50 | 2 μL |
| Sum of antibodies | 4 μL | ||
| 1× Permeabilization Buffer from FoxP3/Transcription Factor Staining Buffer Set | 96 μL | ||
| Final staining volume | 100 μL | ||
