Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Antibody mix for the extracellular staining of thymic T cells
| Fluorophore | Marker | Final dilution | Volume for 1 sample |
|---|---|---|---|
| eFluor 506 | Fixable Viability Dye | 1/200 | 0.5 μL |
| CD16/32 (Mouse BD Fc Block) | 1/100 | 1 μL | |
| APC-eFluor 780 | CD4 | 1/200 | 0.5 μL |
| PE-Cy7 | CD8a | 1/600 | 0.16 μL |
| Sum of antibodies | 2.16 μL | ||
| 1× D-PBS | 97.84 μL | ||
| Final staining volume | 100 μL | ||
