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Antibody mix for the extracellular staining of T cells in the blood of mice
| Fluorophore | Marker | Final dilution | Volume for 1 sample |
|---|---|---|---|
| eFluor 506 | Fixable Viability Dye | 1/200 | 0.5 μL |
| CD16/32 (Mouse BD Fc Block) | 1/100 | 1 μL | |
| PE-Cy5 | CD3e | 1/200 | 0.5 μL |
| APC-eFluor 780 | CD4 | 1/200 | 0.5 μL |
| PE-Cy7 | CD8a | 1/200 | 0.5 μL |
| Alexa Fluor 700 | CD44 | 1/200 | 0.5 μL |
| eFluor 450 | CD62L | 1/200 | 0.5 μL |
| Sum of antibodies | 4 μL | ||
| 1× D-PBS | 96 μL | ||
| Final staining volume | 100 μL | ||
