Table 6.
Antibody mix for the intracellular staining of T cells in the blood of mice
Fluorophore | Marker | Final dilution | Volume for 1 sample |
---|---|---|---|
APC | FoxP3 | 1/50 | 2 μL |
Sum of antibodies | 2 μL | ||
1× Permeabilization Buffer from FoxP3/Transcription Factor Staining Buffer Set | 98 μL | ||
Final staining volume | 100 μL |