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. 2021 Feb 20;2(1):100351. doi: 10.1016/j.xpro.2021.100351

Table 6.

Antibody mix for the intracellular staining of T cells in the blood of mice

Fluorophore Marker Final dilution Volume for 1 sample
APC FoxP3 1/50 2 μL
Sum of antibodies 2 μL
1× Permeabilization Buffer from FoxP3/Transcription Factor Staining Buffer Set 98 μL
Final staining volume 100 μL