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Antibody mix for the extracellular staining of PMA/ionomycin-stimulated splenic T cells
| Fluorophore | Marker | Final dilution | Volume for 1 sample |
|---|---|---|---|
| eFluor 506 | Fixable Viability Dye | 1/200 | 0.5 μL |
| CD16/32 (Mouse BD Fc Block) | 1/100 | 1 μL | |
| PE-Cy5 | CD3e | 1/200 | 0.5 μL |
| BUV395 | CD4 | 1/200 | 0.5 μL |
| FITC | CD8a | 1/200 | 0.5 μL |
| PE | CD44 | 1/200 | 0.5 μL |
| BUV496 | CD45R/B220 | 1/400 | 0.25 μL |
| Sum of antibodies | 3.75 μL | ||
| 1× D-PBS | 46.25 μL | ||
| BD Horizon Brilliant Stain Buffer | 1/2 | 50 μL | |
| Final staining volume | 100 μL | ||
