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Antibody mix for the intracellular staining of PMA/ionomycin-stimulated splenic T cells
| Fluorophore | Marker | Final dilution | Volume for 1 sample |
|---|---|---|---|
| PE-Cy7 | IFN-γ | 1/160 | 0.63 μL |
| APC-Cy7 | IL-2 | 1/200 | 0.5 μL |
| APC | IL-4 | 1/100 | 1 μL |
| PerCP-Cyanine5.5 | IL-17 | 1/160 | 0.63 μL |
| Alexa Fluor 700 | TNF | 1/100 | 1 μL |
| Sum of antibodies | 3.76 μL | ||
| 1× Permeabilization Buffer from BD Cytofix/Cytoperm kit | 96.24 μL | ||
| Final staining volume | 100 μL | ||
