. 2021 Jan 19;7(2):345–354. doi: 10.1021/acscentsci.0c01589

Table 1. Kinetic Parameters for Sialyltransferase-Catalyzed Reaction of CMP 7-Fluorosialic Acid Derivatives.

enzyme	donor	acceptor	K_m (μM)	k_cat (s^–1)	k_cat/K_m (s^–1 mM^–1)
PmST1 (GT80)	CMP-7OHSA	lactose	89 (±1)	180 (±2)	2045
	CMP-7FSA	lactose	52 (±1)	99 (±1)	1900
	CMP-7,7-diFSA	lactose	77 (±1)	10 (±0.3)	130
PsST (GT80)	CMP-7OHSA	lactose	70 (±1)	44 (±1)	656
	CMP-7FSA	lactose	50 (±1)	75 (±1)	1590
CjST1 (GT42)	CMP-7OHSA	lactose	210 (±10)	3.6 (±0.1)	17.3
	CMP-7FSA	lactose	610 (±40)	2.6 (±0.2)	4.2
	CMP-7,7-diFSA	lactose	420 (±40)	1.1 (±0.1)	2.5
CjST2 (GT42)	CMP-7OHSA	3′-sialyllactose	320 (±20)	1.5 (±0.2)	4.8
	CMP-7FSA	3′-sialyllactose	150 (±10)	0.40 (±0.03)	2.6
	CMP-7OHSA	3′-(7F)sialyllactose	210 (±20)	1.3 (±0.01)	6.4
	CMP-7FSA	3′-(7F)sialyllactose	100 (±20)	0.42 (±0.04)	4.3