Figure 6.
Antibody-directed PA conjugates deliver EF and RRSP into EGFR-positive cells. (A) Intracellular cAMP concentrations of MDA-MB-231 cells after 2 h of incubation with 20 nM EF or LFN-DTA and with 100 nM Cmab-mPAC or Tmab-mPAC. Concentrations of cAMP were measured with an ELISA-based competition assay. Data represent the mean ± s.d. of three replicate wells. (B) Western blot analysis of intracellular protein amounts in MDA-MB-231 cells after 24 h of incubation of LFN-RRSP and with 50 nM Cmab-mPAC, Cmab-mPACA, Tmab-mPAC, or a 1:1 mixture of Cmab + mPAC.