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. 2021 Feb 4;7(2):365–378. doi: 10.1021/acscentsci.0c01670

Figure 7.

Figure 7

Protease- and antibody-directed PA conjugates provide dual-targeting selectivity. (A) Cartoon illustrations of IgG-mPAC conjugates cleaved by proteases uPA and MMP-9. (B) SDS-PAGE gel analysis with Western blot visualization of the protease-treated conjugates: Cmab-mPAC, Cmab-mPAC-uPA (Cmab-uPA), and Cmab-mPAC-MMP (Cmab-MMP). (C–F) Relative viability of EGFR-positive cells expressing the uPA and MMP proteases (MDA-MB-231, BT549) after incubation with or without 10 nM LFN-DTA, and with 10-fold serial dilutions of (C, D) Cmab-mPAC-uPA or (E, F) Cmab-mPAC-MMP. Cell viability was determined by the relative luminescence from a CellTiter-Glo assay, which was normalized to untreated cells. Data represent the mean ± s.d. of three replicate wells.