Figure 8.

Antibody-directed PA conjugates enhance biodistribution and pharmacokinetics. (A) Groups of female nude mice i.v. injected with 1 mg/kg ⁸⁹Zr-labeled mPAC-uPA (n = 1), Cmab-mPAC-uPA (n = 4), or Cmab (n = 4). (B) Whole-body PET images and peripheral blood analysis at the indicated time points. (C) Amount in circulation (%) of mPAC-uPA, Cmab-mPAC-uPA, and Cmab based on blood sample radiation measurements using a gamma counter. Data represent the mean ± s.d. of injected dose per gram of tissue (%ID/g), which was normalized to t = 0. The curves represent a two-phase decay model fitted to the data and were used to determine the values for half-life and area under curve. (D) Representative whole-body animal PET images collected at 6 h after injection.