Table 1.
Pyrosequencing assay information.
| Gene | Chromosome Position 1 |
CpG Sites | Primers: Forward (F) Reverse (R) Sequencing (S) |
Sequencing Length |
T° Annealing |
|
|---|---|---|---|---|---|---|
| ARNTL | chr11:13275818 -13278227 |
2 | F R S |
GGGGTTAGTTTGGGTAATAGAATTAG Bio-TAAACTCCCTAAATAAAAAAACAAC TTATTTTATTTTATTTTAGT |
38 bp | 54 °C |
| CLOCK | chr4:55547142 -55547530 |
2 | F R S |
TTTTTAGGAGATGGGAGAAGATGT Bio-TAAAAAATCCAAAAACCAAAAAAAA TTTTTTGTTAATATT |
28 bp | 51.5 °C |
| CRY1 | chr12:105617622 -105618592 |
3 | F R S |
TTTGTGAGGGAAGGTTTAGTTT Bio-AACAATTTCCAAACCCTCC TTTTTAAGGGTTATGAG |
27 bp | 56 °C |
| CRY2 | chr11:45846906 -45847578 |
4 | F R S |
TGTTTTTTGAGATTTGGTTTATTTT Bio- CCAAAACCCCTCTACCATTAACTA TGTTTTTTGAGATTTGGTTTATTTT |
33 bp | 54 °C |
| PER1 | chr17:8151724 -8152661 |
3 | F R S |
TAGGGTTAGGGATTGGAGAATAGA Bio-ACCCAAACAAAAAACACACTATC GGGTTAGGAGTGTAGATTTT |
27 bp | 52 °C |
| PER2 | chr2:238288036 -238291073 |
3 | F R S |
TGAGAAAGGTAGTATTTTTAAGG Bio-AAAACTCCACATACCCCACAC AGGAGGTTGTTTTGGGAGAT |
34 bp | 52 °C |
| PER3 | chr1:7784068 -7785195 |
3 | F R S |
TGTTTGTTATTGATTGTAAAGTGAG Bio-AATTTAAATCCCCCTTTCCCTAC TGTTTGTTATTGATTGTAAAGTGAG |
25 bp | 52 °C |
1 As reported by the UCSC Genome Browser, GRCh38/hg38 assembly.