Table 2.
Ea(HDX) values for WT and Leu343Ala peptides and the resulting ΔEa(HDX) in kcal mol−1a
| Peptide | WT Ea(HDX) | Leu343Ala Ea(HDX) | ΔEa(HDX) | Region | Properties |
|---|---|---|---|---|---|
| 324–333 | 18.9 (0.9) | 7 (1) | −12 (2) | A | TIMb, loop & helix, near Asp320 |
| 345–364 | 14 (2) | 2 (4) | −12 (5) | A | TIMb, loop & helix, near Lys 345 |
| 32–49 | 10.1 (0.4) | 1 (4) | −9 (4) | B | N-terminal L1 |
| 2–23 | 12 (1) | 4 (4) | −8 (4) | B | N-terminal sheet & loop |
| 64–77 | 11 (3) | 5 (2) | −6 (4) | B | N-terminal helix |
| 113–137 | 21 (3) | 6 (5) | −15 (6) | B | N-terminal helix & loop |
| 372–383 | 13 (1) | 11 (1) | −2 (1) | B | TIMb, loop |
| 263–279 | 15 (2) | 2 (4) | −12 (4) | C | TIMb, loop & helix |
| 22–31 | 2 (2) | 7 (2) | 5 (3) | D | N-terminal sheet & loop |
a
ΔEa(HDX) = Ea(HDX)(Leu343Ala) – Ea(HDX)(WT), where ΔEa(HDX) is larger than the error. Values for all Type II peptides are given in Table S6, with four outliers further discussed in SI p6. Activation energies were calculated from the temperature-dependent plots of ln(kHDX) versus inverse temperature (Section S6).
b
TIM refers to the canonical TIM barrel region of enolase. Regions A-D as shown in Figure 8.