Table 1.
Method validation parameters.
| Flavanone | Calibration Range (µM) |
Equation | Linearity (R2) |
R.S.D. 1 (%), n = 6 Within-Day |
R.S.D. (%), n = 6 Between-Day |
LOD 2 (µM) | LOQ 3 (µM) |
Recovery (%) |
|---|---|---|---|---|---|---|---|---|
| Eriocitrin | 0.625–20.0 | y = 19.891x | 0.9993 | 0.177 | 0.177 | 0.014 | 0.042 | 95.28 |
| Neoeriocitrin | 0.625–20.0 | y = 9.627x | 0.9998 | 0.240 | 0.240 | 0.027 | 0.081 | 94.79 |
| Hesperidin | 0.625–20.0 | y = 22.944x | 0.9999 | 0.230 | 0.230 | 0.016 | 0.050 | 99.48 |
| Neohesperidin | 0.625–20.0 | y = 17.954x | 0.9994 | 0.275 | 0.275 | 0.015 | 0.045 | 97.83 |
| Hesperetin | 0.625–20.0 | y = 26.473x | 0.9998 | 0.243 | 0.243 | 0.049 | 0.148 | 90.36 |
1 Relative standard deviation; 2 Limit of detection; 3 Limit of quantification.