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. 2021 Jan 20;10(2):89. doi: 10.3390/pathogens10020089

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the 60-kDa glycoprotein (gp60) gene in Cryptosporidium felis by nested PCR: lanes 1 and 2, SCAU320; lanes 3 and 4, SCAU2396; lanes 5 and 6, SCAU1149; lanes 7 and 8, SCAU1850; lanes 9 and 10, SCAU1851; lanes 11 and 12, SCAU1857; lane M, 100-bp molecular marker; P1 and P2, positive control (C. felis DNA); and N1 and N2, negative control (reagent-grade water).